LOCUS pMOTag4H-crelox-PUR 5028 bp DNA circular 27-MAY-2010 pMOTag4H-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag4H. g>a correction at 2118. Based on pMOTag series made in pBluescript II KS+ Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.  Mol. Biochem. Parasitol. 145, 2005, 117-120. FEATURES Location/Qualifiers rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(2839..2858) /note="T3 Promoter" rep_origin 3325..3892 /note="pUC origin" CDS complement(4040..4900) /note="AMP" promoter complement(2884..3005) /note="lac Promoter" CDS complement(2727..2807) /note="3HA" misc_signal complement(2676..2709) /note="loxP" 5'UTR complement(2563..2648) /function="Procyclin SAS and UTR" /note="The various procyclin SASs differ by only 1 nt, really. This sequence taken from pNS10-54, with hindIII site removed (AAGCTT > AAGACT, leaving splice acceptor AG intact)" misc_feature complement(2505..2562) /function="Residue from original LUC insert" /note="With minor changes to delete the infamous 'upstream ATG' CDS and about 4 deletions/insertions/changes to remove or create restriction sites, compared to the version in pNS10-54, from which Mike got the sequence." CDS complement(1908..2504) /function="PUR" misc_feature complement(1972..2851) /note="verified sequence" /note="g>a correction at 2118." CDS complement(735..1865) /function="HSVTK" CDS complement(1872..1901) /function="Ty-1 epitope" misc_signal complement(695..728) /function="loxP" misc_feature 675..1238 /note="100% verified sequence" ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcCATAACT TCGTATAATG TATGCTATAC 721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG 781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT 841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC 901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG 961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG 1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA 1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA 1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG 1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG 1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC 1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG 1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA 1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT 1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG 1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG 1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT 1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC 1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA 1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG 1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGAGGC ACCGGGCTTG 1921 CGGGTCATGC ACCAAGTGCG CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG 1981 CCGAGCCGCT CGTAGAAGGG GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC 2041 CCGGCGCGCT CGGCCGCCTC CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC 2101 TGGTGGTCGG GCGAGACaCC GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC 2161 GGCGCCAGGA GGCCTTCCAT CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC 2221 ATGCGCGGGC CGATCTCGGC GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG 2281 ACCGCCACTG CTGCGCCGTC GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC 2341 GTGAGGAAGA GTTCTTGCAG CTCGGTGACC CGCTCGATGT GGCGGTCTGG ATCGACGGTG 2401 TGGCGCGTGG CGGGGTAGTC GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCTGGGGACG 2461 TCGTCGCGGG TGGCGAGGCG CACCGTGGGC TTGTACTCGG TCATGGTAGA TCtGTAATGT 2521 CAGTGCGAGC GATCAAGAAT GTAACACAAA GGAATaCCAA GTTTGTGAAT TTTATTTTTT 2581 GGTGTAATTG AAGtCTtcaa gttgaaaaaa atggggaaaa ggaaaaaaaa ctcgaaggcg 2641 cgtgcagcgg ccggccGACG AGAACgTGTa ccggtATAAC TTCGTATAAT GTATGCTATA 2701 CGAAGTTATA AGCTTATCGT CGACTATGCG TAATCGGGCA CATCGTACGG GTATGCGTAG 2761 TCTGGCACGT CGTATGGGTA CGCGTAATCA GGCACATCGT AAGGGTACTC GAgggggggc 2821 ccggtaccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt 2881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 2941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 3001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 3061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 3121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 3661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 3721 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 3781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 3841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 3901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 4021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 4681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 4921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //