LOCUS       pMOTag43-crelox-HYG     5537 bp    DNA     circular     22-APR-2009
pMOTag43M-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag43M.

Based on pMOTag series made in pBluescript II KS+ by Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.  Mol. Biochem. Parasitol. 145, 2005, 117-120.

This file and its predecessor pMOTag43M constructed by George April 2009 using pBluescript II KS+ backbone.
FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(3348..3367)
                     /note="T3 Promoter"
     rep_origin      3834..4401
                     /note="pUC origin"
     CDS             complement(4549..5409)
                     /note="AMP"
     promoter        complement(3393..3514)
                     /note="lac Promoter"
     CDS             complement(3175..3316)
                     /source="3x c-myc"
     CDS             complement(735..1862)
                     /note="HSVTK"
     misc_signal     complement(694..724)
                     /function="loxP"
     CDS             complement(1904..2930)
                     /function="HYG"
     CDS             complement(1872..1901)
                     /function="Ty-1 epitope"
     5'UTR           complement(2997..3082)
                     /function="Procyclin SAS and UTR"
                     /note="The various procyclin SASs differ by onl 1 nt,
                     really. This sequence taken from pNS10-54, with hindIII
                     site removed (AAGCTT > AAGACT, leaving splice acceptor
                     AG intact)"
     misc_feature    complement(2930..2996)
                     /function="Residue from original LUC insert"
                     /note="With minor changes to delete the infamous
                     'upstream ATG' CDS and about 4
                     deletions/insertions/changes to remove or create
                     restriction sites, compared to the version in pNS10-54,
                     from which Mike got the sequence."
     misc_signal     complement(3110..3143)
                     /function="loxP"
     misc_feature    2560..3367
                     /note="100% verified sequence"
BASE COUNT     1276 a   1486 c   1533 g   1242 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgg atcCATAACT TCGTATAATG TATGCTATAC
        721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG
        781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT
        841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC
        901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG
        961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG
       1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA
       1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA
       1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG
       1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG
       1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC
       1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG
       1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA
       1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT
       1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG
       1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG
       1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT
       1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC
       1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA
       1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG
       1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGATTC CTTTGCCCTC
       1921 GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC
       1981 CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG
       2041 ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG
       2101 CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG CGGCGATCCT
       2161 GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC
       2221 GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC
       2281 CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC
       2341 GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA
       2401 GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG
       2461 GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT
       2521 CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATGGCC
       2581 TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA
       2641 CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC AATGTCAAGC
       2701 ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG
       2761 AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG
       2821 AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT
       2881 TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT CATTTACCAA
       2941 CAGTAagatc tGTAATGTCA GTGCGAGCGA TCAAGAATGT AACACAAAGG AATaCCAAGT
       3001 TTGTGAATTT TATTTTTTGG TGTAATTGAA GtCTTCAAGT TGAAAAAAAT GGGGAAAAGG
       3061 AAAAAAAACT CGAAGGCGCG TGCAGCggcc ggccGACGAG AACgTGTacc ggtATAACTT
       3121 CGTATAATGT ATGCTATACG AAGTTATAAG CTTATCGTCG ACGGCCGCAC TAGATCACAG
       3181 CAGATCCTCC TCtGAGATCA GCTTCTGCTC CTCGGATCTC AGCAGATCCT CCTCCGAGAT
       3241 CAGCTTCTGC TCCTCGGATC TCAGCAGATC CTCCTCCGAG ATCAGCTTCT GCTCCTCGGA
       3301 TCTCGATCGG CCGCCCtcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
       3361 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
       3421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
       3481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
       3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
       3601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
       3661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
       3721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
       3781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
       3841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
       3901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
       3961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
       4021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       4081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
       4141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
       4201 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
       4261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
       4321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
       4381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       4441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
       4501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
       4561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
       4621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
       4681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
       4741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
       4801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
       4861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
       4921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
       4981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
       5041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
       5101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
       5161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
       5221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       5281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
       5341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
       5401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
       5461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
       5521 tccccgaaaa gtgccac
//