LOCUS pMOTag2T-crelox-PUR 4976 bp DNA circular 09-APR-2009 pMOTag2T-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag2T made by Luisa. Based on pMOTag series made in pBluescript II KS+ Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.  Mol. Biochem. Parasitol. 145, 2005, 117-120. FEATURES Location/Qualifiers rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(2787..2806) /note="T3 Promoter" rep_origin 3273..3840 /note="pUC origin" CDS complement(3988..4848) /note="AMP" promoter complement(2832..2953) /note="lac Promoter" CDS complement(2725..2757) /note="TY1" misc_signal complement(2676..2709) /function="loxP" 5'UTR complement(2563..2648) /function="Procyclin SAS and UTR" /note="The various procyclin SASs differ by onl 1 nt, really. This sequence taken from pNS10-54, with hindIII site removed (AAGCTT > AAGACT, leaving splice acceptor AG intact)" misc_feature complement(2505..2562) /function="Residue from original LUC insert" /note="With minor changes to delete the infamous 'upstream ATG' CDS and about 4 deletions/insertions/changes to remove or create restriction sites, compared to the version in pNS10-54, from which Mike got the sequence." CDS complement(1908..2504) /function="PUR" /note="Corrected sequence position 1935 a>g and 2118 g>a. Probably same as noted elsewhere in these plasmid PUR sequences, and does not change the amino acids." CDS complement(1872..1901) /function="Ty-1 epitope" CDS complement(735..1865) /note="HSVTK" misc_signal complement(694..728) /function="loxP" misc_feature 630..1380 /note="100% verified sequence" misc_feature 1800..2970 /note="100% verified sequence" BASE COUNT 1107 a 1317 c 1462 g 1090 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atccATAACT TCGTATAATG TATGCTATAC 721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG 781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT 841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC 901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG 961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG 1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA 1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA 1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG 1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG 1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC 1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG 1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA 1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT 1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG 1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG 1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT 1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC 1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA 1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG 1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGAGGC ACCGGGCTTG 1921 CGGGTCATGC ACCAgGTGCG CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG 1981 CCGAGCCGCT CGTAGAAGGG GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC 2041 CCGGCGCGCT CGGCCGCCTC CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC 2101 TGGTGGTCGG GCGAGACaCC GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC 2161 GGCGCCAGGA GGCCTTCCAT CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC 2221 ATGCGCGGGC CGATCTCGGC GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG 2281 ACCGCCACTG CTGCGCCGTC GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC 2341 GTGAGGAAGA GTTCTTGCAG CTCGGTGACC CGCTCGATGT GGCGGTCTGG ATCGACGGTG 2401 TGGCGCGTGG CGGGGTAGTC GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCTGGGGACG 2461 TCGTCGCGGG TGGCGAGGCG CACCGTGGGC TTGTACTCGG TCATGGTAGA TCtGTAATGT 2521 CAGTGCGAGC GATCAAGAAT GTAACACAAA GGAATaCCAA GTTTGTGAAT TTTATTTTTT 2581 GGTGTAATTG AAGtCTTCAA GTTGAAAAAA ATGGGGAAAA GGAAAAAAAA CTCGAAGGCG 2641 CGTGCAGCgg ccggccGACG AGAACgTGTa ccggtATAAC TTCGTATAAT GTATGCTATA 2701 CGAAGTTATA AGCTTATCGT CGACTCAGTC AAGTGGGTCC TGGTTAGTAT GGACCtcgag 2761 ggggggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta 2821 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 2881 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 2941 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3001 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 3061 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 3661 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3841 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 3901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 3961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 4321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 4561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 4621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 4681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 4741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 4801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 4861 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 4921 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac //