LOCUS pLEW20 6800 bp DNA circular 28-JUL-2008 FEATURES Location/Qualifiers misc_feature complement(12..709) /function="rRNA Spacer" promoter 717..965 /function="GPEET promoter" /note="This is more than the minimal promoter -- extends about 145bp upstream of initiation site" protein_bind 971..1010 /function="Two Tet Operators" /note="Operator seq according to Wirtz 1998 paper" 5'UTR 1015..1100 /function="GPEET upstream" 5'UTR 1101..1131 /note="GPEET 5?; UTR" CDS 1163..1204 /function="Extraneous CDS" CDS 1206..2858 /note="LUCIFERASE" 3'UTR 3014..3159 /note="ALD 'short' 3?; UTR" /note="Uses truncated aldolase 3'UTR originally derived from pHD330 (about 6x lower BF expression than the full-length ALD UTR in pLew100, but no difference in expression levels in PF, between long and short UTRs) " 5'UTR 3162..3279 /function="ACT 5?; UTR" CDS 3286..3660 /note="BLE" 3'UTR 3721..4007 /function="ACT 3?; UTR" CDS 4945..5802 /function="LACTAMASE" BASE COUNT 1734 a 1579 c 1732 g 1755 t ORIGIN 1 GAATTCGAGC TCATATAGTT GGTATGTATT CTAATTCCAG ACTACTGGCG TGGATAAACA 61 TGTCCCCTGA TTAAAAGGAA AGATTCCATA GCCCATTAGT GCAAAGATAA TTGGTACACC 121 GTCAAAAACA CATGGGAGAC CACACGATAC GACACTGTGA CCGTAGCATC AATTCCGCAC 181 TCGATATAAC TCTATCGAAG AACTTCCATG GTACAAACTG GTCGCCACGG TCTGCTCCAC 241 ACGGAGATCA TCGTATCATT TTTATCGATA GCGGCCGCTA TCGATGTATG CCTTGGCCCT 301 GATGGCATGC CAATTTCACT ACAACGGACT ATGTGGACCC CGTTATCATG GAAATGCGCT 361 AGTTGGAGGA AGTTAGACCG CGCCGGAAAA GAGAGGGGTA GAGAAAATGA CAACTTGGAA 421 GATATCCACA CACGCACGGT GAAACGTTAG CAACAATTAT TAGGGAAGCA CGCTTGCCCT 481 AGTCCCACGA GTAACCAAGA CTCCAAAAGC CTTTCTGGCA CAGAGAGCGA GCCGAAATGG 541 AAAAGAGAAA CAATGCCTGC ACTAACACTA CTGAGCGATT CGCCTCGCCG CGGAGGACCG 601 AATACTAATA ACGACACTTG CGGTCAAAAA GTAGAAGAAC AAATGCTCAA CGATGAGTGA 661 ATCAGGTTAG GGTAGTTGGA AAATTATACA GAATGTCTTT GGCAACACAC CGGTACCGTC 721 ATTGGGGTTA AGCGGAAAGG TGTGTGTCAG TAGGTTGTGA GGTGAAAGCG TTTTCAGATG 781 CATAGTGAGC TTAATGTCCT TTTCACAGTA TATCGTGTCT GATAGGTATC TCTTATTAGT 841 ATAGTCGAAT ACTAGTCAAT AGTGCGTTTT GTGCAAAATG TCCATTTTGT GGCAGTGATG 901 GGGTTGTTTT ATGCTATTCC GTGTCTCTGG GTGGGCGTGC ATTGAAAATA GGGGTTATCG 961 GGTAGGGATC TCCCTATCAG TGATAGAGAT CTCCCTATCA GTGATAGAGA TCCCTGAGTA 1021 CTGAGTTTAA CATGTTCTCG TCCCGGGCTG CACGCGCCTT CGAGTTTTTT TTCCTTTTCC 1081 CCATTTTTTT CAACTTGAAG ACTTCAATTA CACCAAAAAG TAAAATTCAC AAGCTTGGAA 1141 TTCCTTTGTG TTACATTCTT GAATGTCGCT CGCAGTGACA TTAGCATTCC GGTACTGTTG 1201 GTAAAATGGA AGACGCCAAA AACATAAAGA AAGGCCCGGC GCCATTCTAT CCTCTAGAGG 1261 ATGGAACCGC TGGAGAGCAA CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA 1321 CAATTGCTTT TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA 1381 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT CACAGAATCG 1441 TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT GTTGGGCGCG TTATTTATCG 1501 GAGTTGCAGT TGCGCCCGCG AACGACATTT ATAATGAACG TGAATTGCTC AACAGTATGA 1561 ACATTTCGCA GCCTACCGTA GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG 1621 TGCAAAAAAA ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC 1681 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT TTTAATGAAT 1741 ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT TGCACTGATA ATGAATTCCT 1801 CTGGATCTAC TGGGTTACCT AAGGGTGTGG CCCTTCCGCA TAGAACTGCC TGCGTCAGAT 1861 TCTCGCATGC CAGAGATCCT ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA 1921 GTGTTGTTCC ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG 1981 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA TCCCTTCAGG 2041 ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT TTCATTCTTC GCCAAAAGCA 2101 CTCTGATTGA CAAATACGAT TTATCTAATT TACACGAAAT TGCTTCTGGG GGCGCACCTC 2161 TTTCGAAAGA AGTCGGGGAA GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG 2221 GATATGGGCT CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC 2281 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT CTGGATACCG 2341 GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT CAGAGGACCT ATGATTATGT 2401 CCGGTTATGT AAACAATCCG GAAGCGACCA ACGCCTTGAT TGACAAGGAT GGATGGCTAC 2461 ATTCTGGAGA CATAGCTTAC TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA 2521 AGTCTTTAAT TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT 2581 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT GACGCCGGTG 2641 AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC GATGACGGAA AAAGAGATCG 2701 TGGATTACGT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG 2761 TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC 2821 TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG 2881 ACGAAATTCT TAGCTATTGT AATATTATAT GCAAATTGAT GAATGGTAAT TTTGTAATTG 2941 TGGGTCACTG TACTATTTTA ACGAATAATA AAATCAGGTA TAGGTAACTA AAAAGGAATT 3001 CGAGCTCGGA TCCTGCCCAT TTAGTTGGCT TTTCCCTTGT CTCGTGTCTT TTCCGTGGAA 3061 AGGTTCCCGG AGTAATCTGA TGGCACAGCA GGGAGGTGCG CCTGCAGGTT GGTTAGGAAG 3121 GGGGGATGAT GTAAAAGAAG AAAATGGGGG GATTCGAGCC CGGGCACAGC AAGGTCTTCT 3181 GAAATTCATG TTTTTTTTTT TTTTACTCTG CATTGCAGTC TCCGCTCTTA TTTAGTTTTG 3241 CTTTACGTAA GGTCTCGTTG CTGCCATAAA ATAAGCTATA TCACCATGGC CAAGTTGACC 3301 AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC 3361 CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC 3421 GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG 3481 GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC 3541 TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG 3601 TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA 3661 CCGACGCCGA CCAACACCGC CGGTCCGACG GCGGCCCACG GGTCCCAGGC CTCGGAGATC 3721 CTAACACCGG GTTGTGTGGC CAAAATTGTT CTGTAGTCGC TGTGAGTTGA CACGGCTAGT 3781 GCTTATGATT TTCCTCGCGT GTGGTGCCTG TACTCAGCCC TATGCCTTAT TTGCAACACA 3841 TTTACGTACA GCGCACAAGA GAAGAGAAGA TCACTTGAAG ATAATAAATA TAGGGTTGTA 3901 GGCATCTTGT TTAACTCAAA TTTTCTCGTC TTGGTGTGTC GACATGATTG AAATAGTGCC 3961 ACCAGTTGTG TTTGATGCGT TTGTTATCTA TGCAGTATTC TGCAAGGCCT TGCAGGCATG 4021 CAAGCTAGCT TGTATTCTAT AGTGTCACCT AAATCGTATG TGTATGATAC ATAAGGTTAT 4081 GTATTAATTG TAGCCGCGTT CTAACGACAA TATGTACAAG CCTAATTGTG TAGCATCTGG 4141 CTTACTGAAG CAGACCCTAT CATCTCTCTC GTAAACTGCC GTCAGAGTCG GTTTGGTTGG 4201 ACGAACCTTC TGAGTTTCTG GTAACGCCGT TCCGCACCCC GGAAATGGTC AGCGAACCAA 4261 TCAGCAGGGT CATCGCTAGC CAGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG 4321 GCGCCACAGG TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG 4381 CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG 4441 CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG 4501 CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGATA 4561 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG 4621 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA 4681 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC 4741 GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 4801 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 4861 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 4921 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 4981 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 5041 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 5101 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 5161 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 5221 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 5281 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 5341 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 5401 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 5461 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 5521 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 5581 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 5641 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 5701 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 5761 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 5821 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 5881 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 5941 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 6001 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 6061 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 6121 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 6181 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 6241 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 6301 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 6361 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 6421 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 6481 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6541 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 6601 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 6661 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 6721 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 6781 TGGCCGATTC ATTAATGCAG //