LOCUS pHJ17 6261 bp DNA circular 29-JUL-2013 FEATURES Location/Qualifiers gene 1..905 /note="bTUB gene array" 5'UTR 913..1103 /note="5'UTR ALD" repeat_unit 1109..1142 /note="loxP" misc_difference 1152..1322 /note="procycin SAS" CDS 1326..2348 /note="HYG" primer_bind 1080..1100 /note="PCR-KO forward" misc_feature 2355..2384 /note="Ty1" CDS 2391..3521 /note="HSVTK" repeat_unit 3528..3561 /note="loxP" 3'UTR 3563..3719 /note="3'UTR ALD " primer_bind complement(3565..3585) /note="PCR-KO reverse" CDS 4406..5263 /note="AMP" primer_bind complement(2349..2372) /note="oligo #44" source 1..6261 /dnas_title="pHJ17 (pHJ9 with single NheI)" ORIGIN 1 CTGACCGTAT CATGATGACT TTCTCCATCA TCCCATCCCC CAAGGTGTCC GACACTGTCG 61 TCGAGCCGTA CAATACGACT CTCTCCGTGC ACCAACTTGT GGAAAACTCC GATGAGTCGA 121 TGTGCATTGA CAACGAGGCA CTGTACGATA TTTGCTTCCG CACCCTGAAA CTGACAACAC 181 CAACGTTCGG TGACCTGAAC CACTTGGTGT CTGCTGTTGT GTCCGGCGTC ACCTGCTGCC 241 TGCGCTTCCC TGGTCAGTTG AACTCTGACC TCCGTAAGTT GGCTGTGAAC CTTGTCCCAT 301 TCCCGCGTCT GCACTTCTTC ATGATGGGCT TCGCCCCGCT GACCAGCCGC GGCTCGCAGC 361 AGTACCGCGG TCTCTCCGTG CCCGAGCTAA CGCAGCAGAT GTTCGATGCG AAAAACATGA 421 TGCAAGCTGC AGATCCTCGT CACGGCCGCT ACCTGACAGC GTCTGCACTC TTCCGCGGCC 481 GCATGTCGAC GAAGGAGGTT GATGAGCAGA TGCTGAACGT GCAGAACAAG AACTCGTCCT 541 ACTTCATTGA GTGGATCGAT CCCGAACAAC ATCAAGTCCT CTGTTTGCGA TATCCCACCC 601 AAGGGACTCA AGATGGCTGT CACCTTCATT GGCAACAACA CCTGCATCCA GGAGATGTTC 661 CGCCGTGTGG GAGAGCAGTT CACCCTCATG TTCCGTCGCA AGGCGTTCTT GCACTGGTAC 721 ACTGGCGAGG GTATGGACGA GATGGAATTC ACGGAGGCAG AGTCCAACAT GAACGATCTC 781 GTGTCTGAGT ACCAGCAGTA CCAGGATGCC ACGATTGAGG AGGAGGGCGA GTTCGACGAG 841 GAGGAGCAAT ACTAGACGCG GACGGGGCAT TTCCCGTTCG TCATTAGCAG TAGGTAATGA 901 AGATGCTCGA GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA 961 GGCACGGAAG CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA 1021 ATATTTGCGA TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA 1081 AAACATAAAC TCAACTGCAA CGAAGCTTAT AACTTCGTAT AGCATACATT ATACGAAGTT 1141 ATaccggtAC AcGTTCTCGT Cggccggccg ctgcacgcgc cttcgagttt tttttccttt 1201 tccccatttt tttcaacttg aAGaCTTCAA TTACACCAAA AAATAAAATT CACAAACTTG 1261 GtATTCCTTT GTGTTACATT CTTGATCGCT CGCACTGACA TTACagatct TACTGTTGGT 1321 AAATGATGAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT 1381 TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT 1441 TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA 1501 AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG 1561 ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA 1621 CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA 1681 TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC 1741 AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG 1801 TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG 1861 ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT 1921 TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG 1981 AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT 2041 TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT 2101 CGCCGCGGCT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG 2161 TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT 2221 CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG 2281 ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG 2341 CAAAGGAATC TAGAGAAGTC CATACTAACC AGGACCCACT TGACGCCACC ATGGCCTCGT 2401 ACCCCGGCCA TCAACACGCG TCTGCGTTCG ACCAGGCTGC GCGTTCTCGC GGCCATAGCA 2461 ACCGACGTAC GGCGTTGCGC CCTCGCCGGC AGCAAGAAGC CACGGAAGTC CGCCCGGAGC 2521 AGAAAATGCC CACGCTACTG CGGGTTTATA TAGACGGTCC CCACGGGATG GGGAAAACCA 2581 CCACCACGCA ACTGCTGGTG GCCCTGGGTT CGCGCGACGA TATCGTCTAC GTACCCGAGC 2641 CGATGACTTA CTGGCGGGTG CTGGGGGCTT CCGAGACAAT CGCGAACATC TACACCACAC 2701 AACACCGCCT CGACCAGGGT GAGATATCGG CCGGGGACGC GGCGGTGGTA ATGACAAGCG 2761 CCCAGATAAC AATGGGCATG CCTTATGCCG TGACCGACGC CGTTCTGGCT CCTCATATCG 2821 GGGGGGAGGC TGGGAGCTCA CATGCCCCGC CCCCGGCCCT CACCCTCATC TTCGACCGCC 2881 ATCCCATCGC CGCCCTCCTG TGCTACCCGG CCGCGCGGTA CCTTATGGGC AGCATGACCC 2941 CCCAGGCCGT GCTGGCGTTC GTGGCCCTCA TCCCGCCGAC CTTGCCCGGC ACCAACATCG 3001 TGCTTGGGGC CCTTCCGGAG GACAGACACA TCGACCGCCT GGCCAAACGC CAGCGCCCCG 3061 GCGAGCGGCT GGACCTGGCT ATGCTGGCTG CGATTCGCCG CGTTTACGGG CTACTTGCCA 3121 ATACGGTGCG GTATCTGCAG TGCGGCGGGT CGTGGCGGGA GGACTGGGGA CAGCTTTCGG 3181 GGACGGCCGT GCCGCCCCAG GGTGCCGAGC CCCAGAGCAA CGCGGGCCCA CGACCCCATA 3241 TCGGGGACAC GTTATTTACC CTGTTTCGGG CCCCCGAGTT GCTGGCCCCC AACGGCGACC 3301 TGTATAACGT GTTTGCCTGG GCCTTGGACG TCTTGGCCAA ACGCCTCCGT TCCATGCACG 3361 TCTTTATCCT GGATTACGAC CAATCGCCCG CCGGCTGCCG GGACGCCCTG CTGCAACTTA 3421 CCTCCGGGAT GGTCCAGACC CACGTCACCA CCCCCGGCTC CATACCGACG ATATGCGACC 3481 TGGCGCGCAC GTTTGCCCGG GAGATGGGGG AGGCTAACTG AGAATTCATA ACTTCGTATA 3541 GCATACATTA TACGAAGTTA TGGATCCTGC CCATTTAGTT AGTTGGCTTT TCCCTTGTCT 3601 CGTGTCTTTT CCGTGGAAAG GTTCCCGGAG TAATCTGATG GCACAGCAGG GAGGTGCGCC 3661 TGCAGGTTGG TTAGGAAGGG GGGATGATGT AAAAGAAGAA AATGGGGGGA TTCGAGCCCC 3721 CTTGCAGGCA TGCAAGCTAG CCAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC 3781 GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG 3841 GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG 3901 GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CACCATTCCT TGCGGCGGCG GTGCTCAACG 3961 GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAT 4021 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC 4081 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA 4141 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG 4201 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT 4261 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 4321 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 4381 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 4441 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA 4501 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG 4561 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT 4621 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 4681 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 4741 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC 4801 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 4861 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 4921 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT 4981 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 5041 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 5101 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA 5161 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA 5221 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT 5281 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT 5341 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 5401 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 5461 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 5521 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 5581 TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 5641 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 5701 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 5761 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 5821 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 5881 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 5941 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 6001 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 6061 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA 6121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG 6181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG 6241 TTGGCCGATT CATTAATGCA G //