LOCUS X52327 2961 bp DNA circular SYN 29-JAN-2002 DEFINITION pBluescript II KS(+) vector DNA, phagemid excised from lambda ZAPII. ACCESSION X52327 VERSION X52327.1 GI:58061 KEYWORDS artificial sequence; cloning vector; expression vector; vector. SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 AUTHORS Short,J.M., Fernandez,J.M., Sorge,J.A. and Huse,W.D. TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988) PUBMED 2970625 REFERENCE 2 AUTHORS Alting-Mees,M.A. and Short,J.M. TITLE pBluescript II: gene mapping vectors JOURNAL Nucleic Acids Res. 17 (22), 9494 (1989) PUBMED 2555794 REFERENCE 3 (bases 1 to 2961) AUTHORS Thomas,E.A. TITLE Direct Submission JOURNAL Submitted (20-FEB-1990) Thomas E.A., Stratagene Clonin Systems, 11099 North Torney Pines Rd., La Jolla, CA 92037, USA FEATURES Location/Qualifiers source 1..2961 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" misc_feature 653..760 /note="MCS" CDS complement(460..816) /note="lac Z" /note="Stratagene says this is the Lac Z beta Gal alpha fragment CDS, but the ORF continues to 242." rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(772..791) /note="T3 Promoter" rep_origin 1258..1825 /note="pUC origin" CDS complement(1973..2833) /note="AMP" promoter complement(817..938) /note="lac Promoter" BASE COUNT 747 a 738 c 755 g 721 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa 721 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt 781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 2941 catttccccg aaaagtgcca c //