LOCUS pT13-11_episome 9537 bp DNA circular 23-JUL-2008 Purpose: can be used for episomal expression in PF of a cassette inserted at the EcoN1 site in the plasmid backbone. The plasmid was empirically derived by shotgun cloning genomic EcoRI fragments, so the essential region, designated PMS, is a curious chimera of EcoRI fragments from 3 places in the genome. Junction between PMS and Procyclin promoter region was corrected with info from Pradeep on Jun 8 2006. The origin of the plasmid was Hon's pH51, with the PMS being cloned into the RI site, which was lost in cloning. FEATURES Location/Qualifiers promoter 9512..9531 /function="T7 Promoter in vector" /note="This vector cannot be used in cell lines that express T7 RNAP." CDS 5541..6335 /function="NEO" 3'UTR 6340..6710 /function="GPEET 3' UTR" promoter 4902..5168 /function="GPEET promoter upstream" /note="this is in pH51, in which pT13 was constructed: opened at EcoRI site that was about 10 bp upstream of this sequence, but this was lost in cloning in the PMS." promoter 5169..5440 /function="GPEET promoter" 5'UTR 5445..5498 /function="GPEET SAS" 5'UTR 5499..5540 /function="GPEET 5' UTR" CDS 7641..8498 /function="LACTAMASE" misc_feature 2..4901 /function="PMS" /note="Empirically isolated Plasmid Maintenance Sequence" CDS complement(1..1671) /function="Tb11.47.0035" /note="BLAST identifies this as a calpain-like cysteine peptidase, putative" misc_feature 1890..2126 /note="64% match VSG G4" misc_feature 1762..2709 /note="Unidentified" /note="No match in genome strain" misc_feature 2727..4902 /note="Perfect match on Chr III" misc_feature 3723..5051 /note="Unnecessary for function" CDS 3516..4325 /function="Tb927.3.2670" /note="This region still contains ambiguities in our sequencing, but the TryDB sequence gives a clean ORF annotated as hypothetical conserved." BASE COUNT 2181 a 2391 c 2356 g 2586 t 23 others ORIGIN 1 GAATTCGCGC TCTCTAATTG CTTGTTCTTT GGCAATGTCC AAAATGATAC TGTTAAGCTC 61 CTCCATGATT TCAATAGCAG TTGGGGAATT GTATCTTCCA TCTCGCTTCA ACTCTAGAAG 121 TTCCCTTTCT TTTCCCAAGA ATAATTCACT ATCGTCAACG GGAAGCTCGT CTAGAGTAAA 181 GCCGAGTGGG TGTTGCTCGA GAGATAACCT CACATCACTT CGCAGCTTAC GTGCTATGTC 241 CTCAGAACGT CTCCGCATCG CCCCTGCAAG GTCCTGGATT TTCAAAGTAT CCTTAGCATC 301 AAAAACAGCT TTAATGTATC CATTCTCCAA CTCAATAAAC GGTAAGTCAT TGTCAAGAGG 361 AAGCACCGAC AACGGAAGAC CATCAGGTGA GGTGTCAAGA ACATCCTCTC TCATTTTTCG 421 CTTCACCTCA AGGGCTAAAT CATTCACGTA GTCCCTCATG GCATTTTCCA CGCCCACCAA 481 CTTATGATCA TTCTCGCGAA TGTTCAGACC CTTCAATGTC AAGCGCTCCA CCTCTAGCAA 541 ATGAAAGTTA TCATCATCAT CAAGAGGTAC ACAATGCAAT GGGATGCCAT GAGGCTCAGG 601 ATCGAGAAAC GACCTCTCAC TACGCAACTT TAACAACGCA AGCTTTTCCG CTCGCCCATT 661 CAGGGCSTTC TCCACACTGG CAATTGAGTC CTTGTTACCC ACCACGTCAC GCATTAGCGC 721 AGCTCGTTTC ACTTCAAGCA TCGAAAATTC ATCGTCATTG TCCAGTGGCA CATCATCCAA 781 AGGTACACCA CAAGGGTGAG AAATCAGGTA ATCCTTCCGC TCCGCACAGT TCTTCATTTT 841 AGCAAGCTGA TGCGCACGAC CATCCAGCAA CTCCTCAATG GCAGCAATTT TCTGTGCCTC 901 AGGAGGAGAC TCCTTACAAA GAAGCCGTAG TTGCTTTTCA AGAGAACGAA ACTCCTTATC 961 GCTATCTAGA GGCAATTCCT CCAGTAGACG ACCCTCAGGC TCAGGgAAGA gAAAaGCACG 1021 ATCTTTAGAA ATCATCTCCG CCGCCATCTG CTTGGCTAAC TCGTTCAAGT CAGACTTCAG 1081 CAAAGCAACA TCGGCACCAT TTGCCCGCAG GTCCTTCCGA AGCTTGTGCA GTTCAGCTTC 1141 TTTCGCAACA AAGGTCTCGT TATTGTCAAG GGGCAGCTCA TCTAGGGACA CCCCCAATGG 1201 CATCGGGTCC AGAAACATGC GGATCTCACA ACGCATCTTA TCAGCCAGTT CGATCACCCT 1261 CTCATTCATC GCATCTTCAA GATTTCGTAT CGCTGCTATA TTAGCATGCC GGTCCTCGCA 1321 CAAACCAAAC CGACGAATTT CAAGATCACA AAAAACCTCG TCGTTATCAA TAGGAACATC 1381 CTCAATAGCA ATACCACCAG GGTTAGGGTC CATAAATTTC GATCGCTCCG CGTGCTTCAT 1441 TTCCGTAGCA AGAACGTGTG CACGCTCGTT CAGGCCACCC TCCAAATCCG AGATGGCACG 1501 TCCGTTCCTC TGAGGGTTCT TCTTCAGTAG CGCACGTTTA ATTTCAAGCT CCTGGAACAC 1561 ACTATCGCTA TCAAGTGGTA GCAAACGAAG CGGCACACCA ACAGATGCTG GGTCCAAGTA 1621 ACCACGGTCA CttCGACAAG TGCTCCCTCG CAAGGGCTTC CGCACGAtCa TTCAGTGCaC 1681 GCTCCaCATC CaTTATTACT GTGTCCTGTT TCTTCGCATC ACACTTGAGA CGTTGCCGAT 1741 GCACCTCAAG CTCGTGGAAT TCAGAGCGAA TTTCTTATTG AAAAGAAAAA TTGTATCGTT 1801 TTAAGAGTTA TTATCATTTG CAACCAGCTT CCTTTTTGCA GGCATCTTCC AATTTACCTG 1861 AATATTTCTT TTCCCTCTTC TCATCTTTCC CTCTTTTTTC TTGGTTTTCT TTTTCTATTT 1921 TTTCTTTCAC AGAATTTTTG AACTTGCATT TGTTGGTGTT GAAACTGCAC CCTTTATCAT 1981 CTACTCCCTT ATATTTGACT TTGTTGGTGT CTGCTGAATT GCATTCCTTT TCCTTGTTTT 2041 CTGTTTGTTT TTTCCTTTTG TCATCGCCTC TTGTTGGTTG CAAATTGGAT TCCTCGACCT 2101 GTTGTAGATC TGCCAGATTT TCTTGTGATG CAACCCTTAG TAGCATCTTG AGGTTAGCGA 2161 GTATTTGGGC GTCATGTGCC ACGTTTGCTG CGTGCTGCTC TATGAGTCTT TTCGCTGCTG 2221 ATAACTGTAG CATCCACGGT ATATTGTATG GCATTGAGTC TGCTACGGAT TTGTAAGTGA 2281 TGCAGCCTGC GCCGTTGCTG CTGTCGCAGT TATTGCCTGA TTCTGGTGTG CCGTAAAGCA 2341 CCGCTTTGTG cTCCGCTTCC TCgGTGTTGT TGCCGAGTAA ATCTGTGAAT TGGTGCTGTG 2401 CTGCCGTTAG GTTTGCAGCC TATAATACTT CAATTCGCAG CGGTTTGCAT GTCGCTGCTA 2461 GTCCTGCGTA TTTTTGAGCC GCTGCTGCCG CGTTACTGTG GGTGATAGTT GCCAAGCCGC 2521 CGAATCCTAT GCAACGCTTT TCATAGGCAT TTGTCGAGTC GTGGCTACAG AGGCAGACCG 2581 TATCACTGCC TAATCTTTTG CCTGTACCTC CCAAGGTAGC GCCGTCGGCT TCTTATGCTG 2641 CTCCAGCGCC ATTAGCACTG AATGAGTCTG TTTCTGCTTC TACTTTTACT TCTTTTTTGC 2701 CTGTGGTTAc AGCCGRMAAR TCTCTTTGGT ATCATGCACA TGATGCTGAA CATAGTCCTC 2761 CGATGCGACG GTTTCGAGGA CACACACAAG AGAGTGCCAA GCTCAATGTC AGTTGGGAAG 2821 TTGAAGGCCC TTGTGCGTGC AGTGTATAGC GTGGCTCCGT CCCATCAGCA TTTGTCCTTC 2881 GTGTCAGGTG ATGCTGGAGT GGTAGAGGCA CCGACACAAC TGGATAATGA GCTGCAGAGC 2941 CTTTCATTCT ACGGCATGTG TGACCGGGCC ATAGTACTGG TAGTTGACAC ATCCCTTCGG 3001 AGTTGATGCA TGAGCCTCGC AACACTCCCT GAAGCGAAGC GAAGAGAATT GAAATATTAA 3061 TTCGCAGCCG TGCCACCGCT GCAAAGCAAC CAACCTTATG CAGCTCTCGT TGTTCTTATA 3121 TGATTGATTA CTCTCCGTTT GTGAAACCTT ATGCGCGGTG GTTCCCTGGA GAGCCCTGAT 3181 GATGACGCCA ACTGAGGGTC TTTTCTAGCG GCCGCTTTTC GTAGGGAACA TGCACACGGT 3241 GAGGGGTGAT TTAAGTTGGA AATACGAATG GTAGCGGATA TTCTGTTTTA CTCCACATAG 3301 ATATGAGGCC GCGCGTATAA ATAAATAAAA ATAAATGGGC GTATCTCTCG CTAACCTTCA 3361 ACACAAACAA TATCGGTCCG ACTTCCTTCC TAATATTCGT CGCGCAACCT TYCGGTTTTC 3421 TTTTTTTTTT TTCACGTCTT ATTTAGAACT CGCTTGAACA CAgGGACTTG AATAACTTCA 3481 TTAGCCCAAC GgCGGAGATC CACGTGAGTG ATTTCATGCA GAGACTCTTT TTTAGATCGA 3541 CATGCGCACG TGTCAGATGT GCCGACCTGC GGCTCAAACC ACCGGTATCC ATCCCGCAAG 3601 TGACTTTCCA AACTGTCGCT TTTTCCAGCA GCGCAGCGGG TGCTGCCGTG AGGAGCGGAG 3661 CCGAAGGGAC GAATACAACA ACAAAACGTC GCAGTGGTTT CGGCGGCGGT GCCGGTGGAG 3721 GCGGGCCCGT GAAGGATCGC TATCCGCCCC CAGCAACTGA TCTCGGTGAT CCGCGGCGAC 3781 GCCTGGCCGT GCTTGTGGAT GCCTCAAGTG TTGACCCCCG CGTGTTTCGC AACACAATTC 3841 TGCCAGCAGC GCTGAAGGTT GGCACGCCAG TACTCATACG TATATTTTCG GTGCAGCTGA 3901 GCAGTGAATG GGAACcTCTC GtGGCCGGCA GCGGAGGGAC AAGGGAATCA GCAACCTCAA 3961 CGGAACCGGA GTGGAAGACG TCTGGCACCG CTAATGATGG GAGGGAGGAA GTTAGTGGGG 4021 CCGCGGGGTC TCGCGACAGC GGCGCGTGGT GTCCGCCGGT GGAGTTTTTC AGGGTGGAGC 4081 GCTTCATTCC TGtGScAATG CAAATGKAGK CCGATGCAWA TCATATATTT GWCTTCCGTA 4141 RACAAMACCA GATTKAGSCG GTGGMGTTTG TGTGCGKCKA GGTAGATCKC GGCGTGTWCG 4201 AAGGATTCCT TCCTAATATA GCAGGTAATG GGTTCAATCA ATATGTGTTA GATGAGCTTG 4261 GTATGGCGAA GGAGGTGCTT GAAGATGGAA GATCCGAAGA CGGGACGCCC ACAGTGCAGA 4321 AATAAAAATA ATGGGTGCAA GAAGGCAGAT AATGTGAGGG TTGATGGAGG AGAGGGCTAT 4381 TTAAGAAGGT GAAAGGAGTT TAGAGTACAG CAAGGGAACC GGTGCCAAGT ATTCCTGTGG 4441 AGGAAAGGTG TACCTTTTTG CAGGGTTTCT CATTCCCAGA ATGCACACAC GCACACCCTM 4501 TGCCYTTTCG TATTGTYGSC AGTGGCGTTC GGTAAACGCC ACCGCCGAGC TGTACATGCG 4561 GGAAAGGGGC AGAGGGTTCA TTGTACAATG AGTCGGCGTT ACTTTCTCCC TTTATCCTTC 4621 ACTGCTTGTA ATAAAACCCT CTTTTTGCTT TCTCATAGTC GTGTTCATCC TTATTTCCCC 4681 ACTTTCCGCT GTTCTCTGTT CACTTATGTG CGTGCACATA CGTACATTAT GTGTTGTATT 4741 TTGCGCAACG CCCACGGTAC TAGTAAATGG GATTTGTCGA CCGTGGTGGC GGTCGTGAAG 4801 CGAAGGGGTC GAGCCTACGG CACTCCAAGA AGAACCCTGG CGCCCCTGCT GCCAGAAAGG 4861 CCTCCTCCGT GAAGCAACAA CCACTCGCAC GTGTGGCTCC AGTACCCGGC CTAAATGCCG 4921 CTTGATTCAC CACAAAGTGC TTTTCAGTGC CCCCAAACTG GATTGCCAAA CTTCTGACAA 4981 GTGTATTGTT CCGAGTATGG TGAGCGAATC CCTTCTCTAT TATCAATTCT CGAAAACTCT 5041 TCGGGAGTCA GGGCCCACGG AACCGAGGGG GCGGATGAGG GAGAGGGGGA AACAACAATT 5101 CCGCACCGCG AACAGGCACC GCTCCCCTGG ACCTCTCCCT CAGATGTGAG GTGCAGTCAG 5161 CCTTTGTTGT CATTGGGGTT AAGCGGAAAG GTGTGTGTCA GTAGGTTGTG AGGTGAAAGC 5221 GTTTTCAGAT GCATAGTGAG CTTAATGTCC TTTTCACAGT ATATCGTGTC TGATAGGTAT 5281 CTCTTATTAG TATAGTCGAA TACTAGTCAA TAGTGCGTTT TGTGCAAAAT GTCCATTTTG 5341 TGGCAGTGAT GGGGTTGTTT TATGCTATTC CGTGTCTCTG GGTGGGCGTG CATTGAAAAT 5401 AGGGGTTATC GGGTGAGTAC TGAGTTTAAC ATGTTCTCGT GATCGCTGCA CGCGCCTTCG 5461 AGTTTTTTTT CCTTTTCCCC ATTTTTTTCA ACTTGAAGAC TTCAATTACA CCAAAAAGTA 5521 AAATTCACAA GCTTGTTAAC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG 5581 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG 5641 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT 5701 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG 5761 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT 5821 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT 5881 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG 5941 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG 6001 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC 6061 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC 6121 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG 6181 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG 6241 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA 6301 TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGATCCGG ATCCTAAGCG GATGCAAGCG 6361 TGTAAAGCGC CTCGGAGGAA CGAAACCCCT TTGAAAAGGT GGCTTTCGTT TATATATTCT 6421 CCATCTGGTG CATCGTGTTT ATTTCGTATT ATTCCCTGTC AAAAGCTGCG GATATTCATT 6481 TAATATTCTT TTCGTTATAT ATTTTTAGTT CTATCCTTTA TTTTATCCGT TTTAATCGTC 6541 TTCTGAGACC CACAGCCCTG TAGATTTCTG TGATGTTTCG GTTGCGTATT CCATAATTTT 6601 AAGCGTTTCA CTTCTATTTT TTTTCATTCC TTTGAATTTG GATCTTAAAA TTATCATTGG 6661 TGCCTTGTGT TATTGTGCGT GCTGCGTGTG AATGTGGCGC TCTGCCTGCA GGCATGCAAG 6721 CTAGCTTGTA TTCTATAGTG TCACCTAAAT CGTATGTGTA TGATACATAA GGTTATGTAT 6781 TAATTGTAGC CGCGTTCTAA CGACAATATG TACAAGCCTA ATTGTGTAGC ATCTGGCTTA 6841 CTGAAGCAGA CCCTATCATC TCTCTCGTAA ACTGCCGTCA GAGTCGGTTT GGTTGGACGA 6901 ACCTTCTGAG TTTCTGGTAA CGCCGTTCCG CACCCCGGAA ATGGTCAGCG AACCAATCAG 6961 CAGGGTCATC GCTAGCCAGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC 7021 CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG 7081 CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG 7141 GGGACTGTTG GGCGCCATCT CCTTGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC 7201 AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGATATGGT 7261 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 7321 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG 7381 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA 7441 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 7501 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 7561 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 7621 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 7681 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 7741 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 7801 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 7861 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 7921 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 7981 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 8041 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 8101 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 8161 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 8221 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 8281 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 8341 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 8401 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 8461 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 8521 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 8581 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 8641 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 8701 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 8761 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 8821 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 8881 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 8941 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 9001 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 9061 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 9121 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 9181 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 9241 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 9301 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 9361 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 9421 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 9481 CGATTCATTA ATGCAGCTGG CTTATCGAAA TTAATACGAC TCACTATAGG GAGACCG //