LOCUS pMOTag5H 3798 bp DNA circular 28-MAY-2009 pMOTag5H made by Louise Kemp from her pMOTag2H, replacing PUR with BLE, all based on pMOTag series made in pBluescript II KS+ Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T. A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120. FEATURES Location/Qualifiers rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(1609..1628) /note="T3 Promoter" rep_origin 2095..2662 /note="pUC origin" CDS complement(2810..3670) /note="AMP" promoter complement(1654..1775) /note="lac Promoter" 5'UTR complement(1149..1488) /label=a-b TUB Intergenic /note="I checked this" CDS complement(join(1578..1653,460..689)) /note="lac Z interrupted" /note="Stratagene says this is the Lac Z beta Gal alpha fragment CDS, but the ORF continues to 242." CDS complement(1493..1577) /source="Triple HA" /type="Custom cloned insert" CDS complement(765..1139) /source="BLE" BASE COUNT 965 a 969 c 1005 g 859 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgG ATCCCCCGGG CTGCAGGAAT TCGATCCTCG 721 GACCCGTCGG GCCGCGTCGG ACCGGCGGTG TTGGTCGGCG TCGGTCAGTC CTGCTCCTCG 781 GCCACGAAGT GCACGCAGTT GCCGGCCGGG TCGCGCAGGG CGAACTCCCG CCCCCACGGC 841 TGCTCGCCGA TCTCGGTCAT GGCCGGCCCG GAGGCGTCCC GGAAGTTCGT GGACACGACC 901 TCCGACCACT CGGCGTACAG CTCGTCCAGG CCGCGCACCC ACACCCAGGC CAGGGTGTTG 961 TCCGGCACCA CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA 1021 CCGGCGAAGT CGTCCTCCAC GAAGTCCCGG GAGAACCCGA GCCGGTCGGT CCAGAACTCG 1081 ACCGCTCCGG CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATG 1141 ATCaagcttA AGCTAGCAAT TCGTTTGAAC TATTTTCTTT GATGAAAGGG TTACTATTCT 1201 TTCTCCTCTA GTAGGGCTGG TCGAGAAAAC AAGATTGAAA GAAAAGGCGG AAAGGGAGAG 1261 GGAGAAAGAG GTGTGACACG ACAGGAAGAA GCGAATAAGG GGAAGGAAGG CATACACGCA 1321 ACCACAGACG TGACAGGGTG CCTCCTACAC AAAGGGTGAG CAAAAAAACA AGAAAAACAT 1381 AGGTCCCAAG CGAATTAAAA ACATTTGAGA CAGAAAACAA ATTCACAAAA GAAAGAAAGA 1441 AAGAACATAA ATGAAAACCT ACACATGGTG CGACGTTGTC ACACTTTGtc gaCTATGCGT 1501 AATCGGGCAC ATCGTACGGG TATGCGTAGT CTGGCACGTC GTATGGGTAC GCGTAATCAG 1561 GCACATCGTA AGGGTACtcg agggggggcc cggtacccag cttttgttcc ctttagtgag 1621 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 1681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 1741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 1801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 1861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 1921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 2041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 2281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 2521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 2581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 2641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 2701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 2821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 2881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 2941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 3541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 3601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3781 ttccccgaaa agtgccac //