LOCUS       pMOTag53M                3868 bp    DNA     circular     10-APR-2009
pMOTag53M made by Luisa Figueiredo replacing HYG by BLE in pMOTag43M, based on pMOTag series made in pBluescript II KS+
Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.

FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(1679..1698)
                     /note="T3 Promoter"
     rep_origin      2165..2732
                     /note="pUC origin"
     CDS             complement(2880..3740)
                     /note="AMP"
     promoter        complement(1724..1845)
                     /note="lac Promoter"
     5'UTR           complement(1146..1488)
                     /label=a-b TUB Intergenic
                     /note="I checked this"
     CDS             complement(join(1648..1723,460..689))
                     /note="lac Z interrupted"
                     /note="Stratagene says this is the Lac Z beta Gal alpha
                     fragment CDS, but the ORF continues to 242."
     CDS             complement(1506..1647)
                     /source="Triple c-myc"
     CDS             complement(765..1139)
                     /source="BLE"
BASE COUNT      972 a   1011 c   1010 g    875 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgG ATCcCCCGGG CTGCAGGAAT TCGATCCTCG
        721 GACCCGTCGG GCCGCGTCGG ACCGGCGGTG TTGGTCGGCG TCGGTCAGTC CTGCTCCTCG
        781 GCCACGAAGT GCACGCAGTT GCCGGCCGGG TCGCGCAGGG CGAACTCCCG CCCCCACGGC
        841 TGCTCGCCGA TCTCGGTCAT GGCCGGCCCG GAGGCGTCCC GGAAGTTCGT GGACACGACC
        901 TCCGACCACT CGGCGTACAG CTCGTCCAGG CCGCGCACCC ACACCCAGGC CAGGGTGTTG
        961 TCCGGCACCA CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA
       1021 CCGGCGAAGT CGTCCTCCAC GAAGTCCCGG GAGAACCCGA GCCGGTCGGT CCAGAACTCG
       1081 ACCGCTCCGG CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATG
       1141 ATCaagcttA AGCTAGCAAT TCGTTTGAAC TATTTTCTTT GATGAAAGGG TTACTATTCT
       1201 TTCTCCTCTA GTAGGGCTGG TCGAGAAAAC AAGATTGAAA GAAAAGGCGG AAAGGGAGAG
       1261 GGAGAAAGAG GTGTGACACG ACAGGAAGAA GCGAATAAGG GGAAGGAAGG CATACACGCA
       1321 ACCACAGACG TGACAGGGTG CCTCCTACAC AAAGGGTGAG CAAAAAAACA AGAAAAACAT
       1381 AGGTCCCAAG CGAATTAAAA ACATTTGAGA CAGAAAACAA ATTCACAAAA GAAAGAAAGA
       1441 AAGAACATAA ATGAAAACCT ACACATGGTG CGACGTTGTC ACACTTTGTC GACGGCCGCA
       1501 CTAGATCACA GCAGATCCTC CTCCGAGATC AGCTTCTGCT CCTCGGATCT CAGCAGATCC
       1561 TCCTCCGAGA TCAGCTTCTG CTCCTCGGAT CTCAGCAGAT CCTCCTCCGA GATCAGCTTC
       1621 TGCTCCTCGG ATCTCGATCG GCCGCCCtcg agggggggcc cggtacccag cttttgttcc
       1681 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
       1741 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
       1801 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
       1861 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
       1921 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       1981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
       2041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
       2101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
       2161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
       2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
       2281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
       2341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
       2401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
       2461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
       2521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
       2581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
       2641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
       2701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       2761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
       2821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
       2881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
       2941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
       3001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
       3061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
       3121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
       3181 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
       3241 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
       3301 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
       3361 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
       3421 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
       3481 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
       3541 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
       3601 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
       3661 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
       3721 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
       3781 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
       3841 ccgcgcacat ttccccgaaa agtgccac
//