LOCUS       pMOTag4HM               4569 bp    DNA     circular     19-AUG-2010
pMOTag4HM made by Jessica Wright based on pMOTag43M & pMOTag4H made in pBluescript II KS+
Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.‡ 
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.‡ Mol. Biochem. Parasitol. 145, 2005, 117-120.

Sequence file compiled April 2009 by George.
FEATURES             Location/Qualifiers
     misc_feature    join(1..689,2349..4569)
                     /note="MCS"
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(2380..2399)
                     /note="T3 Promoter"
     rep_origin      2866..3433
                     /note="pUC origin"
     CDS             complement(3581..4441)
                     /note="AMP"
     promoter        complement(2425..2546)
                     /note="lac Promoter"
     CDS             complement(join(2349..2424,460..689))
                     /note="lac Z interrupted"
                     /note="Stratagene says this is the Lac Z beta Gal alpha
                     fragment CDS, but the ORF continues to 242."
     CDS             complement(2185..2343)
                     /gene="3xcMyc"
                     /translation="GGRSRSEEQKLISEEDLLRSEEQKLISEEDLLRSEEQKLISEED
                     LLRSEAAVE"
                     /note="LRSEAAVE linker added before HIS tag"
     CDS             complement(2104..2184)
                     /gene="3xHA"
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
                     /note=" 20100819: corrected two nucleotides (lower case)
                     within the HA tag sequence from sequencing before sending
                     to AddGene"
     5'UTR           complement(1753..2100)
                     /label=a-b TUB intergenic
                     /note="I checked this"
     CDS             complement(715..1740)
                     /note="HYG"
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgG ATCCTTGTTC GGTCGGCATC TACTCTATTC
        721 CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA
        781 GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC
        841 TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC
        901 GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG
        961 CGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA
       1021 AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT
       1081 CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA
       1141 GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG
       1201 CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG
       1261 ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT
       1321 TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC
       1381 ATCCATGGCC TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG
       1441 CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC
       1501 AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG
       1561 ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC
       1621 ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT
       1681 GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT
       1741 CACTAGTTCT AGATCAAGCT tAAGCTAGCA ATTCGTTTGA ACTATTTTCT TTGATGAAAG
       1801 GGTTACTATT CTTTCTCCTC TAGTAGGGCT GGTCGAGAAA ACAAGATTGA AAGAAAAGGC
       1861 GGAAAGGGAG AGGGAGAAMG AGGTGTGACA CGACAGGAAG AAGCGAATAA GGGGAAGGAA
       1921 GGCATACACG CAACCACAGA CGTGACAGGG TGCCTCCTAC ACAAAGGGTG AGCAAAAAAA
       1981 CAAGAAAAAC ATAGGTCCCA AGCGAATTAA AAACATTTGA GACAGAAAAC AAATTCACAA
       2041 AAGAAAGAAA GAAAGAACAT AAATGAAAAC CTACACATGG TGCGACGTTG TCACACTTTG
       2101 TCGACTATGC GTAATCgGGC ACATCGTACG GGTATGCGTA GTCtGGCACG TCGTATGGGT
       2161 ACGCGTAATC AGGCACATCG TAAGGGTAct cgacGGCCGC TTCAGATCGC AGCAGATCCT
       2221 CCTCTGAGAT CAGCTTCTGC TCCTCGGATC TCAGCAGATC CTCCTCCGAG ATCAGCTTCT
       2281 GCTCCTCGGA TCTCAGCAGA TCCTCCTCCG AGATCAGCTT CTGCTCCTCG GATCTCGATC
       2341 GGCCGCCctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg
       2401 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
       2461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
       2521 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
       2581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
       2641 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       2701 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
       2761 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       2821 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
       2881 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
       2941 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
       3001 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
       3061 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
       3121 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
       3181 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
       3241 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
       3301 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
       3361 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
       3421 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
       3481 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
       3541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
       3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
       3661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
       3721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
       3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
       3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
       3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
       3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
       4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
       4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
       4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
       4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
       4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
       4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
       4441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
       4501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
       4561 aagtgccac
//