LOCUS    pMOTag4HM-crelox-HYG       5626 bp    DNA     circular     26-APR-2009

pMOTag4HM-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag4HM made by Jessica.
Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.  Mol. Biochem. Parasitol. 145, 2005, 117-120.

Sequence file compiled April 2009 by George.
FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(3437..3456)
                     /note="T3 Promoter"
     rep_origin      3923..4490
                     /note="pUC origin"
     CDS             complement(4638..5498)
                     /note="AMP"
     promoter        complement(3482..3603)
                     /note="lac Promoter"
     CDS             complement(3243..3401)
                     /gene="3xcMyc"
                     /translation="GGRSRSEEQKLISEEDLLRSEEQKLISEEDLLRSEEQKLISEED
                     LLRSEAAVE"
                     /note="LRSEAAVE linker added before HIS tag"
     CDS             complement(3162..3242)
                     /gene="3xHA"
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(735..1862)
                     /note="HSVTK"
     misc_signal     complement(694..724)
                     /function="loxP"
     CDS             complement(1905..2930)
                     /function="HYG"
     CDS             complement(1872..1901)
                     /function="Ty-1 epitope"
     5'UTR           complement(2997..3082)
                     /function="Procyclin SAS and UTR"
                     /note="The various procyclin SASs differ by onl 1 nt,
                     really. This sequence taken from pNS10-54, with hindIII
                     site removed (AAGCTT > AAGACT, leaving splice acceptor
                     AG intact)"
     misc_feature    complement(2931..2996)
                     /function="Residue from original LUC insert"
                     /note="With minor changes to delete the infamous
                     'upstream ATG' CDS and about 4
                     deletions/insertions/changes to remove or create
                     restriction sites, compared to the version in pNS10-54,
                     from which Mike got the sequence."
     misc_signal     complement(3110..3143)
                     /function="loxP"
     misc_feature    625..1384
                     /note="100% verified sequence"
     misc_feature    2842..3500
                     /note="10% verified sequence"
BASE COUNT     1294 a   1506 c   1562 g   1264 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgg atcCATAACT TCGTATAATG TATGCTATAC
        721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG
        781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT
        841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC
        901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG
        961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG
       1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA
       1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA
       1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG
       1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG
       1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC
       1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG
       1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA
       1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT
       1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG
       1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG
       1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT
       1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC
       1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA
       1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG
       1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGATTC CTTTGCCCTC
       1921 GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC
       1981 CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG
       2041 ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG
       2101 CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG CGGCGATCCT
       2161 GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC
       2221 GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC
       2281 CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC
       2341 GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA
       2401 GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG
       2461 GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT
       2521 CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATGGCC
       2581 TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA
       2641 CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC AATGTCAAGC
       2701 ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG
       2761 AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG
       2821 AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT
       2881 TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT CATTTACCAA
       2941 CAGTAagatc tGTAATGTCA GTGCGAGCGA TCAAGAATGT AACACAAAGG AATaCCAAGT
       3001 TTGTGAATTT TATTTTTTGG TGTAATTGAA GtCTTCAAGT TGAAAAAAAT GGGGAAAAGG
       3061 AAAAAAAACT CGAAGGCGCG TGCAGCggcc ggccGACGAG AACgTGTacc ggtATAACTT
       3121 CGTATAATGT ATGCTATACG AAGTTATAAG CTTATCGTCG ACTATGCGTA ATCTGGCACA
       3181 TCGTACGGGT ATGCGTAGTC gGGCACGTCG TATGGGTACG CGTAATCAGG CACATCGTAA
       3241 GGGTActcga cGGCCGCTTC AGATCGCAGC AGATCCTCCT CTGAGATCAG CTTCTGCTCC
       3301 TCGGATCTCA GCAGATCCTC CTCCGAGATC AGCTTCTGCT CCTCGGATCT CAGCAGATCC
       3361 TCCTCCGAGA TCAGCTTCTG CTCCTCGGAT CTCGATCGGC CGCCctcgag ggggggcccg
       3421 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
       3481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
       3541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
       3601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
       3661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
       3721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
       3781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
       3841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
       3901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
       3961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
       4021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
       4081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
       4141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
       4201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
       4261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
       4321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
       4381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
       4441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
       4501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
       4561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
       4621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
       4681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
       4741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
       4801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
       4861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
       4921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
       4981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
       5041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
       5101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
       5161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
       5221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
       5281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
       5341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
       5401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
       5461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
       5521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
       5581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//