LOCUS       pMOTag4H               4410 bp    DNA     circular     28-MAY-2009

pMOTag5H made by Louise Kemp from her pMOTag2H, replacing PUR with BLE, all based on pMOTag series made in pBluescript II KS+

Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T. A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120

FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(2221..2240)
                     /note="T3 Promoter"
     rep_origin      2707..3274
                     /note="pUC origin"
     CDS             complement(3422..4282)
                     /note="AMP"
     promoter        complement(2266..2387)
                     /note="lac Promoter"
     5'UTR           complement(1761..2100)
                     /label=a-b TUB Intergenic
                     /note="I checked this"
     CDS             complement(join(2190..2265,460..689))
                     /note="lac Z interrupted"
                     /note="Stratagene says this is the Lac Z beta Gal alpha
                     fragment CDS, but the ORF continues to 242."
     CDS             complement(2105..2189)
                     /source="Triple HA"
                     /type="Custom cloned insert"
     CDS             complement(715..1740)
                     /note="HYG"
BASE COUNT     1122 a   1124 c   1146 g   1018 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgG ATCCTTGTTC GGTCGGCATC TACTCTATTC
        721 CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA
        781 GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC
        841 TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC
        901 GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG
        961 CGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA
       1021 AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT
       1081 CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA
       1141 GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG
       1201 CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG
       1261 ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT
       1321 TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC
       1381 ATCCATGGCC TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG
       1441 CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC
       1501 AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG
       1561 ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC
       1621 ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT
       1681 GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT
       1741 CACTAGTTCT AGATCAagct tAAGCTAGCA ATTCGTTTGA ACTATTTTCT TTGATGAAAG
       1801 GGTTACTATT CTTTCTCCTC TAGTAGGGCT GGTCGAGAAA ACAAGATTGA AAGAAAAGGC
       1861 GGAAAGGGAG AGGGAGAAAG AGGTGTGACA CGACAGGAAG AAGCGAATAA GGGGAAGGAA
       1921 GGCATACACG CAACCACAGA CGTGACAGGG TGCCTCCTAC ACAAAGGGTG AGCAAAAAAA
       1981 CAAGAAAAAC ATAGGTCCCA AGCGAATTAA AAACATTTGA GACAGAAAAC AAATTCACAA
       2041 AAGAAAGAAA GAAAGAACAT AAATGAAAAC CTACACATGG TGCGACGTTG TCACACTTTG
       2101 tcgaCTATGC GTAATCGGGC ACATCGTACG GGTATGCGTA GTCTGGCACG TCGTATGGGT
       2161 ACGCGTAATC AGGCACATCG TAAGGGTACt cgaggggggg cccggtaccc agcttttgtt
       2221 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       2281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
       2341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
       2401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
       2461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
       2521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
       2581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
       2641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
       2701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
       2761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
       2821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
       2881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
       2941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
       3001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
       3061 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
       3121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
       3181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
       3241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
       3301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
       3361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
       3421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
       3481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
       3541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
       3601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
       3661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
       3721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
       3781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
       3841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
       3901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
       3961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
       4021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
       4081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
       4141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
       4201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
       4261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
       4321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       4381 ttccgcgcac atttccccga aaagtgccac
//