LOCUS       pMOTag43M                4480 bp    DNA     circular     10-APR-2009
pMOTag43M Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.

This file constructed by George April 2009 using pBluescript II KS+ backbone.

FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(2291..2310)
                     /note="T3 Promoter"
     rep_origin      2777..3344
                     /note="pUC origin"
     CDS             complement(3492..4352)
                     /note="AMP"
     promoter        complement(2336..2457)
                     /note="lac Promoter"
     5'UTR           complement(1761..2100)
                     /label=a-b TUB Intergenic
                     /note="I checked this"
     CDS             complement(join(2260..2335,460..689))
                     /note="lac Z interrupted"
                     /note="Stratagene says this is the Lac Z beta Gal alpha
                     fragment CDS, but the ORF continues to 242."
     CDS             complement(715..1740)
                     /note="HYG"
     CDS             complement(2118..2259)
                     /source="Triple c-myc"
BASE COUNT     1129 a   1166 c   1151 g   1034 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgG ATCCTTGTTC GGTCGGCATC TACTCTATTC
        721 CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA
        781 GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC
        841 TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC
        901 GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG
        961 CGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA
       1021 AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT
       1081 CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA
       1141 GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG
       1201 CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG
       1261 ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT
       1321 TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC
       1381 ATCCATGGCC TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG
       1441 CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC
       1501 AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG
       1561 ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC
       1621 ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT
       1681 GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT
       1741 CACTAGTTCT AGATCAagct tAAGCTAGCA ATTCGTTTGA ACTATTTTCT TTGATGAAAG
       1801 GGTTACTATT CTTTCTCCTC TAGTAGGGCT GGTCGAGAAA ACAAGATTGA AAGAAAAGGC
       1861 GGAAAGGGAG AGGGAGAAAG AGGTGTGACA CGACAGGAAG AAGCGAATAA GGGGAAGGAA
       1921 GGCATACACG CAACCACAGA CGTGACAGGG TGCCTCCTAC ACAAAGGGTG AGCAAAAAAA
       1981 CAAGAAAAAC ATAGGTCCCA AGCGAATTAA AAACATTTGA GACAGAAAAC AAATTCACAA
       2041 AAGAAAGAAA GAAAGAACAT AAATGAAAAC CTACACATGG TGCGACGTTG TCACACTTTG
       2101 TCGACGGCCG CACTAGATCA CAGCAGATCC TCCTCCGAGA TCAGCTTCTG CTCCTCGGAT
       2161 CTCAGCAGAT CCTCCTCCGA GATCAGCTTC TGCTCCTCGG ATCTCAGCAG ATCCTCCTCC
       2221 GAGATCAGCT TCTGCTCCTC GGATCTCGAT CGGCCGCCCt cgaggggggg cccggtaccc
       2281 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
       2341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
       2401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
       2461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
       2521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
       2581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
       2641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
       2701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
       2761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
       2821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
       2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
       2941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       3001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
       3061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
       3121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
       3181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
       3241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
       3301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
       3361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
       3421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
       3481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
       3541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
       3601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
       3661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
       3721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
       3781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
       3841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
       3901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
       3961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
       4021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
       4081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
       4141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
       4201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
       4261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
       4321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
       4381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
       4441 caaatagggg ttccgcgcac atttccccga aaagtgccac
//