LOCUS       pMOTag43M-crelox-PUR    5099 bp    DNA     circular     28-MAY-2009
pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M.

Based on pMOTag series made in pBluescript II KS+ by Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.

This file and its predecessor pMOTag43M constructed by George April 2009 using pBluescript II KS+ backbone.

FEATURES             Location/Qualifiers
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(2910..2929)
                     /note="T3 Promoter"
     rep_origin      3396..3963
                     /note="pUC origin"
     CDS             complement(4111..4971)
                     /note="AMP"
     promoter        complement(2955..3076)
                     /note="lac Promoter"
     CDS             complement(2737..2878)
                     /source="3x c-myc"
     CDS             complement(735..1865)
                     /note="HSVTK"
     misc_signal     complement(694..728)
                     /function="loxP"
     CDS             complement(1908..2504)
                     /function="PUR"
                     /note="Corrected sequence position 1935 a>g and 2118
                     g>a. Probably same as noted elsewhere in these plasmid
                     PUR sequences, and does not change the amino acids."
     CDS             complement(1872..1901)
                     /function="Ty-1 epitope"
     misc_signal     complement(2676..2709)
                     /function="loxP"
     5'UTR           complement(2563..2648)
                     /function="Procyclin SAS and UTR"
                     /note="The various procyclin SASs differ by onl 1 nt,
                     really. This sequence taken from pNS10-54, with hindIII
                     site removed (AAGCTT > AAGACT, leaving splice acceptor
                     AG intact)"
     misc_feature    complement(2505..2562)
                     /function="Residue from original LUC insert"
                     /note="With minor changes to delete the infamous
                     'upstream ATG' CDS and about 4
                     deletions/insertions/changes to remove or create
                     restriction sites, compared to the version in pNS10-54,
                     from which Mike got the sequence."
     misc_feature    1924..2925
                     /note="100% verified sequence"
     misc_feature    679..1380
                     /note="100% verified sequence"
BASE COUNT     1127 a   1370 c   1484 g   1118 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgg atccATAACT TCGTATAATG TATGCTATAC
        721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG
        781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT
        841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC
        901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG
        961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG
       1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA
       1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA
       1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG
       1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG
       1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC
       1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG
       1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA
       1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT
       1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG
       1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG
       1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT
       1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC
       1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA
       1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG
       1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGAGGC ACCGGGCTTG
       1921 CGGGTCATGC ACCAgGTGCG CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG
       1981 CCGAGCCGCT CGTAGAAGGG GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC
       2041 CCGGCGCGCT CGGCCGCCTC CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC
       2101 TGGTGGTCGG GCGAGACaCC GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC
       2161 GGCGCCAGGA GGCCTTCCAT CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC
       2221 ATGCGCGGGC CGATCTCGGC GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG
       2281 ACCGCCACTG CTGCGCCGTC GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC
       2341 GTGAGGAAGA GTTCTTGCAG CTCGGTGACC CGCTCGATGT GGCGGTCTGG ATCGACGGTG
       2401 TGGCGCGTGG CGGGGTAGTC GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCTGGGGACG
       2461 TCGTCGCGGG TGGCGAGGCG CACCGTGGGC TTGTACTCGG TCATGGTAGA TCtGTAATGT
       2521 CAGTGCGAGC GATCAAGAAT GTAACACAAA GGAATaCCAA GTTTGTGAAT TTTATTTTTT
       2581 GGTGTAATTG AAGtCTTCAA GTTGAAAAAA ATGGGGAAAA GGAAAAAAAA CTCGAAGGCG
       2641 CGTGCAGCgg ccggccGACG AGAACgTGTa ccggtATAAC TTCGTATAAT GTATGCTATA
       2701 CGAAGTTATA AGCTTATCGT CGACGGCCGC ACTAGATCAC AGCAGATCCT CCTCtGAGAT
       2761 CAGCTTCTGC TCCTCGGATC TCAGCAGATC CTCCTCCGAG ATCAGCTTCT GCTCCTCGGA
       2821 TCTCAGCAGA TCCTCCTCCG AGATCAGCTT CTGCTCCTCG GATCTCGATC GGCCGCCCtc
       2881 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
       2941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       3001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
       3061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
       3121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
       3181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
       3241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
       3301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
       3361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
       3421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
       3481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
       3541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
       3601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
       3661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
       3721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
       3781 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
       3841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
       3901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
       3961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
       4021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
       4081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
       4141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
       4201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
       4261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
       4321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
       4381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
       4441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
       4501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
       4561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
       4621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
       4681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
       4741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
       4801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
       4861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
       4921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
       4981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
       5041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//