LOCUS pMOTag2T 3899 bp DNA circular 13-APR-2009 pMOTag2T made by Luisa Figueiredo based on pMOTag series made in pBluescript II KS+ Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120 Partial sequencing (march 2009) of PUR showed a>g at position 725 and g>a at 908, with no amino acid change. FEATURES Location/Qualifiers rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(1710..1729) /note="T3 Promoter" rep_origin 2196..2763 /note="pUC origin" CDS complement(2911..3771) /note="AMP" promoter complement(1755..1876) /note="lac Promoter" CDS complement(1651..1680) /note="TY1" CDS complement(695..1294) /note="PUR" 5'UTR complement(1298..1642) /label=a-b TUB Intergenic /note="I checked this" primer_bind complement(1678..1698) /note="Primer NS105" primer_bind 667..692 /note="Primer NS106" primer_bind 563..581 /note="Primer pMOTdown" primer_bind complement(1019..1036) /note="Primer 122puro" BASE COUNT 960 a 991 c 1074 g 874 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgG ATCcTCAGGC ACCGGGCTTG CGGGTCATGC 721 ACCAgGTGCG CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT 781 CGTAGAAGGG GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT 841 CGGCCGCCTC CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG 901 GCGAGACaCC GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA 961 GGCCTTCCAT CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC 1021 CGATCTCGGC GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACTG 1081 CTGCGCCGTC GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA 1141 GTTCTTGCAG CTCGGTGACC CGCTCGATGT GGCGGTCTGG ATCGACGGTG TGGCGCGTGG 1201 CGGGGTAGTC GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCTGGGGACG TCGTCGCGGG 1261 TGGCGAGGCG CACCGTGGGC TTGTACTCGG TCATGGTaag cttAAGCTAG CAATTCGTTT 1321 GAACTATTTT CTTTGATGAA AGGGTTACTA TTCTTTCTCC TCTAGTAGGG CTGGTCGAGA 1381 AAACAAGATT GAAAGAAAAG GCGGAAAGGG AGAGGGAGAA AGAGGTGTGA CACGACAGGA 1441 AGAAGCGAAT AAGGGGAAGG AAGGCATACA CGCAACCACA GACGTGACAG GGTGCCTCCT 1501 ACACAAAGGG TGAGCAAAAA AACAAGAAAA ACATAGGTCC CAAGCGAATT AAAAACATTT 1561 GAGACAGAAA ACAAATTCAC AAAAGAAAGA AAGAAAGAAC ATAAATGAAA ACCTACACAT 1621 GGTGCGACGT TGTCACACTT TGTCGAcTCA GTCAAGTGGG TCCTGGTTAG TATGGACCtc 1681 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc 1741 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 1801 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 1861 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 1921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 1981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 2041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 2101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 2161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 2221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 2281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 2341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 2401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 2461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 2521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 2581 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 2641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 2701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 2761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 2821 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 2881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 2941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 3001 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 3061 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 3121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 3181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 3241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 3301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 3361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 3421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 3481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 3541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 3601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 3661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 3721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 3781 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 3841 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac //