LOCUS pMOTag2T-crelox-HYG 5414 bp DNA circular 09-APR-2009 pMOTag2T-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag2T made by Luisa. Based on pMOTag series made in pBluescript II KS+ Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.  A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes.  Mol. Biochem. Parasitol. 145, 2005, 117-120. FEATURES Location/Qualifiers rep_origin complement(135..440) /note="f1 origin of ss-DNA replication" promoter 625..644 /note="T7 Promoter" promoter complement(3225..3244) /note="T3 Promoter" rep_origin 3711..4278 /note="pUC origin" CDS complement(4426..5286) /note="AMP" promoter complement(3270..3391) /note="lac Promoter" misc_signal complement(3110..3143) /function="loxP" 5'UTR complement(2997..3082) /function="Procyclin SAS and UTR" /note="The various procyclin SASs differ by onl 1 nt, really. This sequence taken from pNS10-54, with hindIII site removed (AAGCTT > AAGACT, leaving splice acceptor AG intact)" misc_feature complement(2930..2996) /function="Residue from original LUC insert" /note="With minor changes to delete the infamous 'upstream ATG' CDS and about 4 deletions/insertions/changes to remove or create restriction sites, compared to the version in pNS10-54, from which Mike got the sequence." CDS complement(1904..2929) /function="HYG" misc_feature complement(2274..3978) /note="100% verified sequence" CDS complement(1872..1901) /function="Ty-1 epitope" CDS complement(731..1861) /note="HSVTK" misc_signal complement(694..724) /function="loxP" CDS complement(3166..3195) /note="Ty-1" /note="According to paper, epitope is EVHTNQDPLD" misc_feature 628..1400 /note="100% verified sequence" BASE COUNT 1256 a 1433 c 1511 g 1214 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcCATAACT TCGTATAATG TATGCTATAC 721 GAAGTTATGA ATTCTCAGTT AGCCTCCCCC ATCTCCCGGG CAAACGTGCG CGCCAGGTCG 781 CATATCGTCG GTATGGAGCC GGGGGTGGTG ACGTGGGTCT GGACCATCCC GGAGGTAAGT 841 TGCAGCAGGG CGTCCCGGCA GCCGGCGGGC GATTGGTCGT AATCCAGGAT AAAGACGTGC 901 ATGGAACGGA GGCGTTTGGC CAAGACGTCC AAGGCCCAGG CAAACACGTT ATACAGGTCG 961 CCGTTGGGGG CCAGCAACTC GGGGGCCCGA AACAGGGTAA ATAACGTGTC CCCGATATGG 1021 GGTCGTGGGC CCGCGTTGCT CTGGGGCTCG GCACCCTGGG GCGGCACGGC CGTCCCCGAA 1081 AGCTGTCCCC AGTCCTCCCG CCACGACCCG CCGCACTGCA GATACCGCAC CGTATTGGCA 1141 AGTAGCCCGT AAACGCGGCG AATCGCAGCC AGCATAGCCA GGTCCAGCCG CTCGCCGGGG 1201 CGCTGGCGTT TGGCCAGGCG GTCGATGTGT CTGTCCTCCG GAAGGGCCCC AAGCACGATG 1261 TTGGTGCCGG GCAAGGTCGG CGGGATGAGG GCCACGAACG CCAGCACGGC CTGGGGGGTC 1321 ATGCTGCCCA TAAGGTACCG CGCGGCCGGG TAGCACAGGA GGGCGGCGAT GGGATGGCGG 1381 TCGAAGATGA GGGTGAGGGC CGGGGGCGGG GCATGTGAGC TCCCAGCCTC CCCCCCGATA 1441 TGAGGAGCCA GAACGGCGTC GGTCACGGCA TAAGGCATGC CCATTGTTAT CTGGGCGCTT 1501 GTCATTACCA CCGCCGCGTC CCCGGCCGAT ATCTCACCCT GGTCGAGGCG GTGTTGTGTG 1561 GTGTAGATGT TCGCGATTGT CTCGGAAGCC CCCAGCACCC GCCAGTAAGT CATCGGCTCG 1621 GGTACGTAGA CGATATCGTC GCGCGAACCC AGGGCCACCA GCAGTTGCGT GGTGGTGGTT 1681 TTCCCCATCC CGTGGGGACC GTCTATATAA ACCCGCAGTA GCGTGGGCAT TTTCTGCTCC 1741 GGGCGGACTT CCGTGGCTTC TTGCTGCCGG CGAGGGCGCA ACGCCGTACG TCGGTTGCTA 1801 TGGCCGCGAG AACGCGCAGC CTGGTCGAAC GCAGACGCGT GTTGATGGCC GGGGTACGAG 1861 GCCATGGTGG CGTCAAGTGG GTCCTGGTTA GTATGGACTT CTCTAGATTC CTTTGCCCTC 1921 GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC 1981 CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG 2041 ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG 2101 CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGCCG CGGCGATCCT 2161 GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC 2221 GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC 2281 CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC 2341 GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA 2401 GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG 2461 GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT 2521 CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATGGCC 2581 TCCGCGACCG GCTGCAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA 2641 CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TGAATTCCCC AATGTCAAGC 2701 ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG 2761 AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG 2821 AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT 2881 TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT CATTTACCAA 2941 CAGTAagatc tGTAATGTCA GTGCGAGCGA TCAAGAATGT AACACAAAGG AATaCCAAGT 3001 TTGTGAATTT TATTTTTTGG TGTAATTGAA GtCTTCAAGT TGAAAAAAAT GGGGAAAAGG 3061 AAAAAAAACT CGAAGGCGCG TGCAGCggcc ggccGACGAG AACgTGTacc ggtATAACTT 3121 CGTATAATGT ATGCTATACG AAGTTATAAG CTTATCGTCG ACtcaGTCAA GTGGGTCCTG 3181 GTTAGTATGG ACCtcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt 3241 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 3301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 3361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 3421 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 3481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 3841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 4021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 4081 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 4141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 4201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 4381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 4441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 4501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 4561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 4621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 4681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 4741 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 4801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 4861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 4921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 5041 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 5101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 5161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 5221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 5281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 5341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 5401 ccgaaaagtg ccac //