LOCUS       pMOTag2H                3952 bp    DNA     circular     28-MAY-2009

pMOTag2H made by Louise Kemp from Luisa Figueiredo's pMOTag2T, replacing the Ty1 tag with the triple HA tag, all based on pMOTag series made in pBluescript II KS+
Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T. A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120
FEATURES             Location/Qualifiers
     misc_feature    join(1..689,1732..3952)
                     /note="MCS"
     rep_origin      complement(135..440)
                     /note="f1 origin of ss-DNA replication"
     promoter        625..644
                     /note="T7 Promoter"
     promoter        complement(1763..1782)
                     /note="T3 Promoter"
     rep_origin      2249..2816
                     /note="pUC origin"
     CDS             complement(2964..3824)
                     /note="AMP"
     promoter        complement(1808..1929)
                     /note="lac Promoter"
     CDS             complement(695..1294)
                     /note="PUR"
     5'UTR           complement(1298..1642)
                     /label=a-b TUB Intergenic
                     /note="I checked this"
     CDS             complement(join(1732..1807,460..689))
                     /note="lac Z interrupted"
                     /note="Stratagene says this is the Lac Z beta Gal alpha
                     fragment CDS, but the ORF continues to 242."
     CDS             complement(1647..1731)
                     /source="Triple HA"
                     /type="Custom cloned insert"
BASE COUNT      973 a   1003 c   1091 g    885 t
ORIGIN      
          1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
         61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
        121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
        181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
        241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
        301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
        361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
        421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
        481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
        541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
        601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
        661 ccgcggtggc ggccgctcta gaactagtgG ATCcTCAGGC ACCGGGCTTG CGGGTCATGC
        721 ACCAAGTGCG CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT
        781 CGTAGAAGGG GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT
        841 CGGCCGCCTC CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG
        901 GCGAGACGCC GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA
        961 GGCCTTCCAT CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC
       1021 CGATCTCGGC GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACTG
       1081 CTGCGCCGTC GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA
       1141 GTTCTTGCAG CTCGGTGACC CGCTCGATGT GGCGGTCTGG ATCGACGGTG TGGCGCGTGG
       1201 CGGGGTAGTC GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCTGGGGACG TCGTCGCGGG
       1261 TGGCGAGGCG CACCGTGGGC TTGTACTCGG TCATGGTaag cttAAGCTAG CAATTCGTTT
       1321 GAACTATTTT CTTTGATGAA AGGGTTACTA TTCTTTCTCC TCTAGTAGGG CTGGTCGAGA
       1381 AAACAAGATT GAAAGAAAAG GCGGAAAGGG AGAGGGAGAA AGAGGTGTGA CACGACAGGA
       1441 AGAAGCGAAT AAGGGGAAGG AAGGCATACA CGCAACCACA GACGTGACAG GGTGCCTCCT
       1501 ACACAAAGGG TGAGCAAAAA AACAAGAAAA ACATAGGTCC CAAGCGAATT AAAAACATTT
       1561 GAGACAGAAA ACAAATTCAC AAAAGAAAGA AAGAAAGAAC ATAAATGAAA ACCTACACAT
       1621 GGTGCGACGT TGTCACACTT TGtcgaCTAT GCGTAATCGG GCACATCGTA CGGGTATGCG
       1681 TAGTCTGGCA CGTCGTATGG GTACGCGTAA TCAGGCACAT CGTAAGGGTA Ctcgaggggg
       1741 ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
       1801 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
       1861 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
       1921 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
       1981 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
       2041 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
       2101 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
       2161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
       2221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
       2281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
       2341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
       2401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
       2461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
       2521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
       2581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
       2641 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
       2701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
       2761 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
       2821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
       2881 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
       2941 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
       3001 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
       3061 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
       3121 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
       3181 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
       3241 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
       3301 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
       3361 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
       3421 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
       3481 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
       3541 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
       3601 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
       3661 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
       3721 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
       3781 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
       3841 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
       3901 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//