LOCUS plew90 6188 bp DNA circular 16-MAR-2010 FEATURES Location/Qualifiers misc_feature join(5713..6188,1..427) /function="%DF TUB Target" promoter 464..483 /function="10% T7 promoter" /note="10% mutant T7 promoter and initiation region. Cloned in filled Mlu of ald sas as annealed oligo cassette with filled Age overhangs" 5'UTR 434..669 /function="ALD SAS & 5' UTR" /note="aldolase-derived splice acceptor site" 3'UTR 1304..1448 /function="ALD 3' UTR" /note="truncated ald 3'UTR derived from HD360" 5'UTR 1455..1567 /function="ACT SAS" /note="actin-derived sas" CDS 1575..2610 /function="HYG" /note="Hygromycin resistance gene derived from HD330" 3'UTR 2628..2918 /function="ACT 3' UTR" CDS 3857..4713 /function="LACTAMASE" promoter 464..483 /function="10% T7 promoter" CDS 674..1297 /function="Tet repressor" /note="CDS corrected 091022 to match the actually sequenced (November 2007) version in pLEW29, which is also a perfect match for sequences in GenBank (checked 091022)" ORIGIN 1 GGCCGCATGT CGACGAAGGA GGTTGATGAG CAGATGCTGA ACGTGCAGAA CAAGAACTCG 61 TCCTACTTCA TTGAGTGGAG ATCCCGAACA ACATCAAGTC CTCTGTTTGC GATATCCCAC 121 CCAAGGGACT CAAGATGGCT GTCACCTTCA TTGGCAACAA CACCTGCATC CAGGAGATGT 181 TCCGCCGTGT GGGAGAGCAG TTCACCCTCA TGTTCCGTCG CAAGGCGTTC TTGCACTGGT 241 ACACTGGCGA GGGTATGGAC GAGATGGAAT TCACGGAGGC AGAGTCCAAC ATGAACGATC 301 TCGTGTCTGA GTACCAGCAG TACCAGGATG CCACGATTGA GGAGGAGGGC GAGTTCGACG 361 AGGAGGAGCA ATACTAGACG CGGACGGGGC ATTTCCCGTT CGTCATTAGC AGTAGGTAAT 421 GAAGATGCTC GAGGGTGCTC AAGCTGTGTA GCGCACGCGC CGGTAATACG TCTCACTATA 481 GGGAGATCTA CCGGCGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA GCCTAACAAA 541 TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG ATAGCTTAGC 601 TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA CTCAACTGCA 661 ACGAAGCTTT CATATGTCTA GATTAGATAA AAGTAAAGTG ATTAACAGCG CATTAGAGCT 721 GCTTAATGAG GTCGGAATCG AAGGTTTAAC AACCCGTAAA CTCGCCCAGA AGCTAGGTGT 781 AGAGCAGCCT ACATTGTATT GGCATGTAAA AAATAAGCGG GCTTTGCTCG ACGCCTTAGC 841 CATTGAGATG TTAGATAGGC ACCATACTCA CTTTTGCCCT TTAGAAGGGG AAAGCTGGCA 901 AGATTTTTTA CGTAATAACG CTAAAAGTTT TAGATGTGCT TTACTAAGTC ATCGCGATGG 961 AGCAAAAGTA CATTTAGGTA CACGGCCTAC AGAAAAACAG TATGAAACTC TCGAAAATCA 1021 ATTAGCCTTT TTATGCCAAC AAGGTTTTTC ACTAGAGAAT GCATTATATG CACTCAGCGC 1081 TGTGGGGCAT TTTACTTTAG GTTGCGTATT GGAAGATCAA GAGCATCAAG TCGCTAAAGA 1141 AGAAAGGGAA ACACCTACTA CTGATAGTAT GCCGCCATTA TTACGACAAG CTATCGAATT 1201 ATTTGATCAC CAAGGTGCAG AGCCAGCCTT CTTATTCGGC CTTGAATTGA TCATATGCGG 1261 ATTAGAAAAA CAACTTAAAT GTGAAAGTGG GTCTTAAGGA TCCTGCCCAT TTAGTTGGCT 1321 TTTCCCTTGT CTCGTGTCTT TTCCGTGGAA AGGTTCCCGG AGTAATCTGA TGGCACAGCA 1381 GGGAGGTGCG CCTGCAGGTT GGTTAGGAAG GGGGGATGAT GTAAAAGAAG AAAATGGGGG 1441 GATTCGAGCC CGGGCACAGC AAGGTCTTCT GAAATTCATG TTTTTTTTTT TTTTACTCTG 1501 CATTGCAGTC TCCGCTCTTA TTTAGTTTTG CTTTACGTAA GGTCTCGTTG CTGCCATAAA 1561 ATAAGCTACT AGTGATGAAA AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA 1621 TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG 1681 CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG 1741 GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG 1801 AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC 1861 AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTG CAGCCGGTCG 1921 CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT 1981 TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG 2041 ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC 2101 AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC 2161 ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG 2221 ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA 2281 GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC 2341 TTGCAGGATC GCCGCGGCTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC 2401 AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA 2461 TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG 2521 TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC CCCAGCACTC 2581 GTCCGAGGGC AAAGGAATAG AGTAGATGCC GACCGGGATC GATCCCCCGA TCCTAACACC 2641 GGGTTGTGTG GCCAAAATTG TTCTGTAGTC GCTGTGAGTT GACACGGCTA GTGCTTATGA 2701 TTTTCCTCGC GTGTGGTGCC TGTACTCAGC CCTATGCCTT ATTTGCAACA CATTTACGTA 2761 CAGCGCACAA GAGAAGAGAA GATCACTTGA AGATAATAAA TATAGGGTTG TAGGCATCTT 2821 GTTTAACTCA AATTTTCTCG TCTTGGTGTG TCGACATGAT TGAAATAGTG CCACCAGTTG 2881 TGTTTGATGC GTTTGTTATC TATGCAGTAT TCTGCAAGGC CTTGCAGGCA TGCAAGCTAG 2941 CTTGTATTCT ATAGTGTCAC CTAAATCGTA TGTGTATGAT ACATAAGGTT ATGTATTAAT 3001 TGTAGCCGCG TTCTAACGAC AATATGTACA AGCCTAATTG TGTAGCATCT GGCTTACTGA 3061 AGCAGACCCT ATCATCTCTC TCGTAAACTG CCGTCAGAGT CGGTTTGGTT GGACGAACCT 3121 TCTGAGTTTC TGGTAACGCC GTTCCGCACC CCGGAAATGG TCAGCGAACC AATCAGCAGG 3181 GTCATCGCTA GCCAGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA 3241 GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC 3301 TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA 3361 CTGTTGGGCG CCATCTCCTT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC 3421 TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA TATGGTGCAC 3481 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC 3541 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC 3601 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG 3661 AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 3721 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 3781 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 3841 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 3901 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 3961 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 4021 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 4081 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 4141 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 4201 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 4261 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 4321 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 4381 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 4441 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 4501 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 4561 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 4621 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 4681 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 4741 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 4801 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 4861 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 4921 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 4981 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 5041 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 5101 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 5161 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 5221 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 5281 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 5341 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 5401 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 5461 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 5521 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 5581 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 5641 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 5701 TCATTAATGC AGCTGACCGT ATCATGATGA CTTTCTCCAT CATCCCATCC CCCAAGGTGT 5761 CCGACACTGT CGTCGAGCCG TACAATACGA CTCTCTCCGT GCACCAACTT GTGGAAAACT 5821 CCGATGAGTC GATGTGCATT GACAACGAGG CACTGTACGA TATTTGCTTC CGCACCCTGA 5881 AACTGACAAC ACCAACGTTC GGTGACCTGA ACCACTTGGT GTCTGCTGTT GTGTCCGGCG 5941 TCACCTGCTG CCTGCGCTTC CCTGGTCAGT TGAACTCTGA CCTCCGTAAG TTGGCTGTGA 6001 ACCTTGTCCC ATTCCCGCGT CTGCACTTCT TCATGATGGG CTTCGCCCCG CTGACCAGCC 6061 GCGGCTCGCA GCAGTACCGC GGTCTCTCCG TGCCCGAGCT AACGCAGCAG ATGTTCGATG 6121 CGAAAAACAT GATGCAAGCT GCAGATCCTC GTCACGGCCG CTACCTGACA GCGTCTGCAC 6181 TCTTCCGC //