LOCUS pLEW111 4705 bp DNA circular 17-JUL-2008 FEATURES Location/Qualifiers misc_feature complement(join(4698..4705,1..712)) /function="RDNA Spacer" promoter 719..738 /function="T7 Promoter" /note="T7 promoter & initiation region. Bgl II starts @ +4. 1 TetR Operator in Bgl II. Promoter-Op-Luc cassette orig from Lew19" CDS 744..762 /function="Tet Operator" /note="2 class B TetR Operator" 5'UTR 769..824 /function="GPEET SAS" /note="to splice site" 5'UTR 825..855 /note="GPEET 5´ UTR" 3'UTR 899..1051 /note="ALD 3´ UTR" 5'UTR 1052..1166 /function="ACT 5' UTR & SAS" /note="actin-derived sas; used in all HD330, 430 & Lew5 descendants" CDS 1176..1547 /function="BLE" 3'UTR 1602..1889 /function="ACT 3´ UTR" /note="actin 3'UTR- same as what's used in all HD330, 430 and Lew5 descendants" /note="perfect match with 3480 to 3612 in X52586" /note="truncated aldolase 3'UTR originally derived from pHD330 (about 6x lower BF expression than the full-length ALD UTR in pLew100, but no difference in expression levels in PF, between long and short UTRs) " terminator 1890..2030 /function="T7 Term" /note="Pet3a fragment (originally PCR'd), then subcloned from published HD216, bearing T7 terminator" terminator 2031..2171 /function="T7 Term" /note="Pet3a fragment (originally PCR'd then subcloned from published HD216) bearing T7 terminator" CDS 2850..3707 /function="LACTAMASE" BASE COUNT 1139 a 1182 c 1247 g 1137 t ORIGIN 1 GAATTCGAGC TCATATAGTT GGTATGTATT CTAATTCCAG ACTACTGGCG TGGATAAACA 61 TGTCCCCTGA TTAAAAGGAA AGATTCCATA GCCCATTAGT GCAAAGATAA TTGGTACACC 121 GTCAAAAACA CATGGGAGAC CACACGATAC GACACTGTGA CCGTAGCATC AATTCCGCAC 181 TCGATATAAC TCTATCGAAG AACTTCCATG GTACAAACTG GTCGCCACGG TCTGCTCCAC 241 ACGGAGATCA TCGTATCATT TTTATCGATA GCGGCCGCTA TCGATGTATG CCTTGGCCCT 301 GATGGCATGC CAATTTCACT ACAACGGACT ATGTGGACCC CGTTATCATG GAAATGCGCT 361 AGTTGGAGGA AGTTAGACCG CGCCGGAAAA GAGAGGGGTA GAGAAAATGA CAACTTGGAA 421 GATATCCACA CACGCACGGT GAAACGTTAG CAACAATTAT TAGGGAAGCA CGCTTGCCCT 481 AGTCCCACGA GTAACCAAGA CTCCAAAAGC CTTTCTGGCA CAGAGAGCGA GCCGAAATGG 541 AAAAGAGAAA CAATGCCTGC ACTAACACTA CTGAGCGATT CGCCTCGCCG CGGAGGACCG 601 AATACTAATA ACGACACTTG CGGTCAAAAA GTAGAAGAAC AAATGCTCAA CGATGAGTGA 661 ATCAGGTTAG GGTAGTTGGA AAATTATACA GAATGTCTTT GGCAACACAC CGGCTGATTA 721 ATACGACTCA CTATAGGGAG ATCTCCCTAT CAGTGATAGA GATCTCCCGG GCTGCACGCG 781 CCTTCGAGTT TTTTTTCCTT TTCCCCATTT TTTTCAACTT GAAGACTTCA ATTACACCAA 841 AAAGTAAAAT TCACAAGCTT CGTCGCGACC AGTGTGCTGG CTGCTGGCCA GCACACTGGA 901 TCCTGCCCAT TTAGTTGGCT TTTCCCTTGT CTCGTGTCTT TTCCGTGGAA AGGTTCCCGG 961 AGTAATCTGA TGGCACAGCA GGGAGGTGCG CCTGCAGGTT GGTTAGGAAG GGGGGATGAT 1021 GTAAAAGAAG AAAATGGGGG GATTCGAGCC CGGGCACAGC AAGGTCTTCT GAAATTCATG 1081 TTTTTTTTTT TTTTACTCTG CATTGCAGTC TCCGCTCTTA TTTAGTTTTG CTTTACGTAA 1141 GGTCTCGTTG CTGCCATAAA ATAAGCTATA TCACCATGGC CAAGTTGACC AGTGCCGTTC 1201 CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT 1261 TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT 1321 TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC 1381 GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG 1441 CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC 1501 GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA CCGACGCCGA 1561 CCAACACCGC CGGTCCGACG GCGGCCCACG GGTCCCAGGC CTCGGAGATC CTAACACCGG 1621 GTTGTGTGGC CAAAATTGTT CTGTAGTCGC TGTGAGTTGA CACGGCTAGT GCTTATGATT 1681 TTCCTCGCGT GTGGTGCCTG TACTCAGCCC TATGCCTTAT TTGCAACACA TTTACGTACA 1741 GCGCACAAGA GAAGAGAAGA TCACTTGAAG ATAATAAATA TAGGGTTGTA GGCATCTTGT 1801 TTAACTCAAA TTTTCTCGTC TTGGTGTGTC GACATGATTG AAATAGTGCC ACCAGTTGTG 1861 TTTGATGCGT TTGTTATCTA TGCAGTATTC TGCAGGCTGC TAACAAAGCC CGAAAGGAAG 1921 CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC 1981 GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATAGGGC TGCTAACAAA 2041 GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT 2101 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAG 2161 GGCCCCTGCA GGCATGCAAG CTAGCCAGAT CCTCTACGCC GGACGCATCG TGGCCGGCAT 2221 CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA 2281 TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC 2341 CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCACCAT TCCTTGCGGC GGCGGTGCTC 2401 AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT 2461 CGATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC 2521 ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA 2581 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA 2641 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 2701 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 2761 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 2821 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 2881 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 2941 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 3001 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA 3061 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 3121 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 3181 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 3241 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 3301 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 3361 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 3421 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 3481 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 3541 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 3601 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 3661 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 3721 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 3781 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 3841 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 3901 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 3961 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 4021 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 4081 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 4141 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 4201 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 4261 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 4321 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 4381 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 4441 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 4501 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 4561 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 4621 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 4681 CGCGTTGGCC GATTCATTAA TGCAG //