The Trypanosoma cruzi insect
stage-specific antigen GP72 was purified from epimastigotes and the
amino acid sequences of peptide fragments determined.
Oligonucleotides derived from these data were used to amplify and
clone a cDNA sequence, which was used to isolate a full-length gene.
All the sequenced peptides were encoded within the gene. The
characteristics of the encoded 62600-Da protein, including a
potential amino-terminal signal sequence, a hydrophobic
carboxy-terminus, and a large number of potential O-glycosylation
sites, are consistent with the properties of GP72. No sequence
homologies were found in searches of DNA and protein data banks. GP72
is encoded by a single pair of non-telomeric allelic genes. (C) 1991
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